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Test Code (รหัสการทดสอบ):
MIBTS0020

Order Name (ชื่อการทดสอบ):
Gram Stain, Sputum

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Sputum/ Sterile container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hour.
 
Useful For (ประโยชน์การทดสอบ):
Screening sputum specimens for acceptability for bacterial culture Identifying microorganisms in sputum Guiding initial antimicrobial therapy
 
Methodology (วิธีการทดสอบ):
Conventional Gram Stain Procedure
 
AliasesName (ชื่อเรียกอื่นๆ) :
Sputum Gram Stain
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0020

Order Name (ชื่อการทดสอบ):
Gram Stain, Sputum

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Sputum/ Sterile container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Sputum, volume Entire collection (minimum 0.5 mL.)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Swab in Stuart transport medium will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Sputum Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0020

Order Name (ชื่อการทดสอบ):
Gram Stain, Sputum

 
Method detail (วิธีการทดสอบ):
Conventional Gram Stain Procedure
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hour.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days for specimen, 1 month for gram stain slide
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0020

Order Name (ชื่อการทดสอบ):
Gram Stain, Sputum

 
 
Clinical Information (ข้อมูลทางคลินิก):
The Gram stain is a general stain used extensively in microbiology for the preliminary differentiation of microbiological organisms. The Gram stain is one of the simplest, least expensive, and most useful of the rapid methods used to identify and classify bacteria. The Gram stain is used to provide preliminary information concerning the type of organisms present directly from clinical specimens or from growth on culture plates. This stain is used to identify the presence of microorganisms in normally sterile body fluids (cerebrospinal fluid, synovial fluid, pleural fluid, peritoneal fluid). It is also used to screen sputum specimens to establish acceptability for bacterial culture (<25 squamous epithelial cells per field is considered an acceptable specimen for culture) and may reveal the causative organism in bacterial pneumonia.
 
Reference Value (ค่าอ้างอิง):
No microorganisms seen
 
Interpretation (การแปลผล):
Positive Gram stain results usually include a description of what was seen on the slide. This typically includes: Whether the bacteria are Gram-positive (purple) or Gram-negative (red) Shape — round (cocci) or rods (bacilli) Size, relative quantity (CFU/mL), and/or arrangement of the bacteria Whether there are bacteria present within other cells (intracellular) Presence white blood cells Fungi (in the form of yeasts or molds) may be seen on a Gram stain and are reported.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)