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Clear
 
Test Code (รหัสการทดสอบ):
MIBTS0040

Order Name (ชื่อการทดสอบ):
Gram Stain, Vagina

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Vaginal swab/ Slide container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours.
 
Useful For (ประโยชน์การทดสอบ):
Supporting the diagnosis of bacterial vaginosis
 
Methodology (วิธีการทดสอบ):
Conventional Gram Stain Procedure and Nugent Scoring System
 
AliasesName (ชื่อเรียกอื่นๆ) :
Gram stain Gram Vagina
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0040

Order Name (ชื่อการทดสอบ):
Gram Stain, Vagina

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Vaginal swab/ Slide container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Smear on Microscopic Slide
 
Sub Mission Container (ภาชนะส่งตรวจ):
Slide container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Swab in Stuart transport medium will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Vaginal smear Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0040

Order Name (ชื่อการทดสอบ):
Gram Stain, Vagina

 
Method detail (วิธีการทดสอบ):
Conventional Gram Stain Procedure and Nugent Scoring System
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
1 month
 
 
 
Test Code (รหัสการทดสอบ):
MIBTS0040

Order Name (ชื่อการทดสอบ):
Gram Stain, Vagina

 
 
Clinical Information (ข้อมูลทางคลินิก):
Bacterial vaginosis is so-named because bacteria are the cause and an associated inflammatory response is lacking. It results in an increase in thin, gray, homogeneous vaginal discharge and vaginal malodor and is caused by a change in the vaginal flora. Bacterial vaginosis is a synergistic polymicrobial infection not caused by a specific organism. The standard scoring system termed the "Nugent score" is a technique for assessing bacterial vaginosis using microscopic examination of a Gram-stained smear of vaginal discharge.
 
Reference Value (ค่าอ้างอิง):
One of the 3 following reports dependent on the weighted sum balance of Lactobacillus, Gardnerella/Bacteroides, and Mobiluncus species:
  1. Consistent with normal bacterial vaginal flora.
  2. Altered vaginal flora not consistent with bacterial vaginosis. This frequently represents a intermediate stage. If signs or symptoms persist, repeat testing is warranted.
  3. Consistent with bacterial vaginosis.
 
Interpretation (การแปลผล):
Assessment of a Gram-stained slide using the Nugent score has replaced culture as the preferred test to diagnose bacterial vaginosis. While Gardnerella is the most common anaerobe found in bacterial vaginosis, other anaerobic organisms are often present along with a decrease in the amount of "usual flora" (eg, Lactobacillus species). This system uses a 0- to 4-point scale to calculate the weighted sum of the following 3 bacterial morphotypes: Lactobacillus, Gardnerella/Bacteroides, and Mobiluncus species. Total score of 0 to 3 is considered normal. Total score of 4 to 6 is considered intermediate stage Total score of 7-10 is considered Bacterial vaginosis.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. ashington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)