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Test Code (รหัสการทดสอบ):
090-40-0070-01

Order Name (ชื่อการทดสอบ):
Bone Marrow C/S

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Bone marrow/ Bactec Plus Aerobic/ F Vial
* Prepare puncture site as for surgical incision.
 
Turnaround Time (ระยะเวลารอผล):
Preliminary Report: Received specimen to preliminary report within 2 days.
Final Report: Received specimen to final report within 5 days.
 
Useful For (ประโยชน์การทดสอบ):
An aid in the diagnosis of bacterial infections
 
Methodology (วิธีการทดสอบ):
1. Continuously Monitored Bone marrow culture Automated Instrument:
    BD BactecTM FX (fluorescence detection technic)
2. Conventional Culture Technique for positive signal
3. Identification by MALDI-TOF
4. Suceptibility test by Vitek 2 Compact
 
AliasesName (ชื่อเรียกอื่นๆ) :
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0070-01

Order Name (ชื่อการทดสอบ):
Bone Marrow C/S

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Medications: Do not use antibiotics before collection of specimen that cause sepsis.
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Bone marrow/ Bactec Plus Aerobic/ F Vial
* Prepare puncture site as for surgical incision.
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Bone marrow, Bactec Plus Aerobic/F Vial volume 8-10 mL (minimum volume 3 mL)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Bactec Plus Aerobic/F Vial (for adult); Gray top, plastic vial
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Refrigerated specimen will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Bone marrow Room temperature, 18oC to 25oC 2 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0070-01

Order Name (ชื่อการทดสอบ):
Bone Marrow C/S

 
Method detail (วิธีการทดสอบ):
1. Continuously Monitored Bone marrow culture Automated Instrument:
    BD BactecTM FX (fluorescence detection technic)
2. Conventional Culture Technique for positive signal
3. Identification by MALDI-TOF
4. Suceptibility test by Vitek 2 Compact
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Preliminary Report: Received specimen to preliminary report within 2 days.
Final Report: Received specimen to final report within 5 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
5 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0070-01

Order Name (ชื่อการทดสอบ):
Bone Marrow C/S

 
 
Clinical Information (ข้อมูลทางคลินิก):
Bone marrow culture is an examination of the soft, fatty tissue found inside certain bones. The bone marrow tissue produces blood cells. This test is done to look for an infection inside the bone marrow. Bone marrow cultures are commonly obtained in the evaluation of fever of unknown origin, particularly for patients who are immunosuppressed. In the effort to identify the cause of systemic infection, BMCs are often performed in conjunction with blood cultures and cultures of other body fluids and tissues
 
Reference Value (ค่าอ้างอิง):
No growth after 5 days
 
Interpretation (การแปลผล):
Positive cultures of Bacteria and yeast are reported with the organism identification and susceptiblity testing. Positive cultures are usually an indication of infection and are reported as soon as detected. Correlation of culture results and the clinical situation is required for optimal patient management. A final negative report is issued after 5 days of incubation.
 
Clinical Reference (เอกสารอ้างอิง):
1. Manufacturer’s Reagent package insert, BD BactecTM FX
2. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition.
    Washington DC: American Society for Microbiology; 2016
3. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th Edition.
    Washington DC: ASM Press; 2015
​4. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)