bih.button.backtotop.text
BROWSE BY TEST NAME
#
%
1
2
3
5
8
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Z
Clear
 
Test Code (รหัสการทดสอบ):
090-10-1161

Order Name (ชื่อการทดสอบ):
PTT mixing study

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to report within 1 hour
 
Useful For (ประโยชน์การทดสอบ):
Determining the cause of a prolonged PT, factor deficiency versus factor inhibitor such as Lupus anticoagulant or Antibody to Factor VIII in Hemophilia A and Hemophilia B pateint.
 
Methodology (วิธีการทดสอบ):
Clotting assay
 
AliasesName (ชื่อเรียกอื่นๆ) :
APTT Mix 1:1
APTTM
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1161

Order Name (ชื่อการทดสอบ):
PTT mixing study

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Citrate plasma, minimum volume 0.5 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: Hemoglobin >500 mg/dL
Lipemia: Triglyceride > 1,000 mg/dL
Icterus: Bilirubin >26 mg/dL
Other: Clotted specimen will be rejected.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Whole Blood, Sodium citrate Room temperature, 18oC to 25oC 4 hours
Plasma, Sodium citrate Room temperature, 18oC to 25oC 4 hours
Refrigerated, 2oC to 8oC 4 hours
Frozen, -20oC 2 weeks
Frozen, -70oC 1 year
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1161

Order Name (ชื่อการทดสอบ):
PTT mixing study

 
Method detail (วิธีการทดสอบ):
Clotting assay
 
Schedule (ตารางการทดสอบ):
Tested Daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to report within 1 hour
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Hematology, Laboratory department, Te. 17254
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
14 days
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1161

Order Name (ชื่อการทดสอบ):
PTT mixing study

 
 
Clinical Information (ข้อมูลทางคลินิก):
The activated partial thromboplastin time (APTT) mix is only performed when the APTT is abnormally prolonged. Please refer to test APTTB / Activated Partial Thromboplastin Time (APTT), Plasma for interpretation of results.

The APTT mixing test is used to evaluate a prolonged APTT test result, especially when mixing test results are combined with results of other coagulation tests and clinical information, to assist in differentiating coagulation factor deficiencies from coagulation inhibitors.
 
Interpretation (การแปลผล):
The APTT mixing study, using equal volumes of patient and normal pool plasma, may be performed on specimens with a prolonged APTT to assist in differentiating coagulation factor deficiencies from coagulation inhibitors of all types (1-4). Correction of the APTT mix to within the normal reference range usually indicates a coagulation factor deficiency (normal plasma in the mixture ensures at least 50% activity of all coagulation factors). If the prolonged APTT is due to an inhibitor (eg, specific coagulation factor inhibitor, lupus anticoagulant, heparin), the APTT mix typically fails to correct a prolonged APTT. However, the presence of a weak inhibitor may be missed by the APTT mixing study.

Accurate interpretation of both APTT and APTT mixing study results may often require additional testing. For example, the thrombin time (TT) test is helpful for identifying or excluding the presence of heparin, the platelet neutralization procedure (PNP, using a modified APTT method) for identifying or excluding lupus anticoagulant, the prothrombin time (PT) and dilute Russell's viper venom time (DRVVT) for further assessment of the common procoagulant pathway, and coagulation factor assays to detect and identify deficient or abnormal factors. These assays are available as components of reflexive and interpretive testing panels in the Special Coagulation Laboratory (eg, PROCT / Prolonged Clot Time Profile).

Shortening of the APTT usually reflects either elevation of factor VIII activity secondary to acute or chronic illness or inflammation, or spurious results from suboptimal venipuncture, specimen collection or processing. A normal or shortened APTT result does not exclude a hemostatic defect; and specific clotting factor assays should be performed despite a normal APTT when there is clinical impression of bleeding diathesis.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s reagent package insert, SynthASil, HemosIL™, Instrumentation Laboratory Company - Bedford, MA 01730-2443 (USA), Instrumentation Laboratory SpA-V.le Monza 338 - 20128 Milano (Italy); 02/2016
  2. https://www.mayocliniclabs.com (Retrieved: 22 Jan 2019)