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Test Code (รหัสการทดสอบ):
AT3

Order Name (ชื่อการทดสอบ):
Antithrombin III

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 2 tubes
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to reported within 4 days
 
Useful For (ประโยชน์การทดสอบ):
Exclude or diagnose hereditary deficiency in patients with a tendency toward thromboembolism, in pre-operative stages, before prescription of oral contraceptives, DIC, nephrotic syndromes, liver diseases and in therapy with heparin or antithrombin concentrates.
 
Methodology (วิธีการทดสอบ):
Chromogenic substrate
 
AliasesName (ชื่อเรียกอื่นๆ) :
Antithrombin Activity
AT3 Activity/Functional
AT III Activity/Functional
Anti-Thrombin III
 
 
 
Test Code (รหัสการทดสอบ):
AT3

Order Name (ชื่อการทดสอบ):
Antithrombin III

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 2 tubes
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Citrate plasma, minimum volume 0.5 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: Hemoglobin > 500 mg/dL
Lipemia: Triglyceride > 2,300 mg/dL
Icterus: Bilirubin > 40 mg/dL
Other: Clotted specimen will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Whole blood Room temperature, 18oC to 25oC 4 hours
Platelet-poor plasma Room temperature, 18oC to 25oC 4 hours
Refrigerated, 2oC to 8oC 4 hours
Frozen, -20oC 2 weeks
Frozen, -70oC 1 year
 
 
 
Test Code (รหัสการทดสอบ):
AT3

Order Name (ชื่อการทดสอบ):
Antithrombin III

 
Method detail (วิธีการทดสอบ):
Chromogenic substrate
 
Schedule (ตารางการทดสอบ):
Tested every Tuesday and Friday.
Note: If not delivered at the time of testing, will be done in the next round
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to reported within 4 days
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
14 days
 
 
 
Test Code (รหัสการทดสอบ):
AT3

Order Name (ชื่อการทดสอบ):
Antithrombin III

 
 
Clinical Information (ข้อมูลทางคลินิก):
Antithrombin (AT) or Heparin Cofactor I is the major inhibitor of blood coagulation and is essential for effective heparin therapy. By inhibiting the coagulation proteases, especially thrombin, FXa and FIXa, AT prevents uncontrolled coagulation and thrombosis. Antithrombin deficiency is associated with a high risk of thromboembolic disorders.

The Liquid Antithrombin kit is an assay based on a synthetic chromogenic substrate and on FXa inactivation Antithrombin levels in patient plasma are measured automatically in two stages:
  1. Incubation of the plasma with the Factor Xa reagent in the presence of an excess of heparin.
  2. Quantification of the residual FXa activity with a synthetic chromogenic substrate.  The paranitroaniline released is monitored kinetically at 405 nm and is inversely proportional to the Antithrombin level in the test sample.
 
Reference Value (ค่าอ้างอิง):
83-128 % Activity
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s reagent Package Insert, Liquid Antithrombin HemosIL®, Instrumentation Laboratory Company - Bedford, MA 01730-2443 (USA), Instrumentation Laboratory SpA- V.le Monza 338 - 20128 Milano (Italy), 08/2014
  2. https://www.mayocliniclabs.com (Retrieved: 22 Jan 2019)