090-30-2790

25-OH Vitamin D, Total

Blood/ Plain blood (Red top) 6 mL, 1 tube

Serum, minimum volume 2 mL

Plastic vial

Hemolysis: 4+ reject.
Lipemia: 4+ reject.
Icterus: 4+ reject.

Specimen Type	Temperature	Time
Serum (in original tube)	Room Temperature	< 72 hours
Serum	Refrigerated, 2oC to 8oC	12 days
	Frozen, -20oC	12 months

090-30-2790

25-OH Vitamin D, Total

Chemiluminescent Microparticle Immuno Assay (CMIA)

Tested daily (24 hours)

Specimen collected to reported within 2 Hours 

Immunology, Laboratory Department Tel. 13227

5 days

090-30-2790

25-OH Vitamin D, Total

Vitamin D is a fat-soluble steroid prohormone mainly produced photochemically in the skin from 7-dehydrocholesterol. Two forms of vitamin D are biologically relevant - vitamin D3 (Cholecalciferol) and vitamin D2 (Ergocalciferol). Both vitamins D3 and D2 can be absorbed from food, with vitamin D2 being an artificial source, but only an estimated 10-20% of vitamin D is supplied through nutritional intake. 

Vitamins D3 and D2 can be found in vitamin supplements. Vitamin D is converted to the active hormone 1,25-(OH)2-vitamin D (Calcitriol) through two hydroxylation reactions. The first hydroxylation converts vitamin D into 25-OH vitamin D and occurs in the liver. The second hydroxylation converts 25-OH vitamin D into the biologically active 1,25-(OH)2-vitamin D and occurs in the kidneys as well as in many other cells of the body. Most cells express the vitamin D receptor and about 3% of the human genome is directly or indirectly regulated by the vitamin D endocrine system.

The major storage form of vitamin D is 25-OH vitamin D and is present in the blood at up to 1,000 fold higher concentration compared to the active 1,25-(OH)2-vitamin D. 25-OH vitamin D has a half-life of 2-3 weeks vs. 4 hours for 1,25-(OH)2-vitamin D. Therefore, 25-OH vitamin D is the analyte of choice for determination of the vitamin D status. Epidemiological studies have shown a high global prevalence of vitamin D insufficiency and deficiency. Risk factors for vitamin D deficiency include low sun exposure, malnutrition, some malabsorption syndromes, and liver or kidney diseases. The measurement of vitamin D status provides opportunities for
preventive and therapeutic interventions. Vitamin D deficiency is a cause of secondary hyperparathyroidism
and diseases resulting in impaired bone metabolism (like rickets, osteoporosis, osteomalacia) 

	Concentration in serum (ng/mL)
Optimal	>= 30
Insufficiency	21 - 29
Deficiency	<= 20
Toxicity possible	> 100

1. Manufacturer’s Reagent package insert, 25-OH Vitamin D,  Abbott Ireland Diagnostic Division Lisnamuck, Longford Co. Longford, Ireland.
2. (*) Reference range: Reference range from The Endocrine Society.
3. (*) Holick MF et al. Evaluation, treatment and prevention of vitamin D defieciency: an endocrine society clinical practice guideline. J Clin Endocrine Metab 2011. 96(7) 1911-1930
4. http://www.mayomedicallaboratories.com (Retrieved: 01 Jun 2020)