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Test Code (รหัสการทดสอบ):
BNP

Order Name (ชื่อการทดสอบ):
BNP (Brain Natriuretic Peptide)*

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Specimen collected to reported within 2 hours
 
 
Useful For (ประโยชน์การทดสอบ):
Determination of human B-type natriuretic peptide (BNP) in human EDTA plasma.

Used as an aid in the diagnosis and assessment of severity of heart failure.
 
Methodology (วิธีการทดสอบ):
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
AliasesName (ชื่อเรียกอื่นๆ) :
Brain Natriuretic Peptide
 
 
 
Test Code (รหัสการทดสอบ):
BNP

Order Name (ชื่อการทดสอบ):
BNP (Brain Natriuretic Peptide)*

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
K3EDTA Plasma, minimum volume 1 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic vial
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Whole blood EDTA (keep in original tube) Refrigrtated, 2oC to 8oC 4 hours
Plasma EDTA Refrigerated, 2oC to 8oC 1 day
Frozen, - 20oC 3 months
 
 
 
Test Code (รหัสการทดสอบ):
BNP

Order Name (ชื่อการทดสอบ):
BNP (Brain Natriuretic Peptide)*

 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Specimen collected to reported within 2 hours
 
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Immunology, Laboratory Department Tel. 13227
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
5 days
 
 
 
Test Code (รหัสการทดสอบ):
BNP

Order Name (ชื่อการทดสอบ):
BNP (Brain Natriuretic Peptide)*

 
 
Clinical Information (ข้อมูลทางคลินิก):
Heart failure is a syndrome caused by a variety of conditions such as coronary artery disease, hypertension, valve disease, myocarditis and others. Common symptoms of heart failure include shortness of breath, coughing under exertion, swelling of appendages, and dizziness. Heart failure is better defined as the progressive inability of the heart ventricles to pump blood out to the lungs and/or the extremities. Heart failure is
either systolic or diastolic or a combination of both. Severity is usually classified into four classes defined by the New York Heart Association (NYHA class I-IV).

BNP is one member of the family of natriuretic peptides that were initially discovered by de Bold, et al. Although BNP was first isolated from porcine brain tissue (originally named brain natriuretic peptide), the heart has been determined to be the major source. BNP is synthesized and released into the blood in response to volume overload or conditions that cause ventricular stretch, to control fluid and electrolyte homeostasis by interaction with the renin-angiotensin-aldosterone system (RAAS).

PreproBNP (134 amino acids) is synthesized in the cardiac myocytes and is processed to a proBNP (108 amino acids) precursor molecule. TheproBNP is subsequently cleaved into physiologically active BNP (32 aminoacids), and a degradation fragment NT-proBNP (76 amino acids).  BNP, NT-proBNP, and a higher molecular weight form have been detected in peripheral blood. BNP is cleared from the circulation, with a half-life (t½) of approximately 23 minutes, by specific cellular receptors and neutral endopeptidases. Numerous studies have indicated that BNP can be used for patient diagnosis, prognosis and therapy monitoring. Levels of BNP have been shown to be elevated in patients with cardiac dysfunction.

Plasma BNP levels provide clinically useful information concerning the diagnosis and management of left ventricular dysfunction and heart failure, which complements other diagnostic testing procedures (e.g.,
electrocardiograms, chest x-rays, and echocardiograms). BNP levels can be used to assess the severity of heart failure, as demonstrated by the correlation with New York Heart Association classifications. Plasma BNP
levels also increase with decreasing physiological functional capacities, as measured by left ventricular ejection fraction (LVEF) or exercise-based evaluations. The European Society of Cardiology has included the use of natriuretic peptides (e.g., BNP) testing in their guidelines for the diagnosis or rule out of heart failure.
        Others have suggested that BNP has utility in the stratification of patients with heart failure and acute coronary syndrome (ACS). Elevated levels of BNP in heart failure patients predict disease progression and increased morbidity and mortality. Studies also suggest ACS patients with increased BNP levels have a higher rate of cardiac complications and higher mortality post myocardial infarction. Preliminary studies have reported the use of BNP measurements to optimize patient treatment / management for heart failure. Nesiritide (Natrecor), recombinant BNP has been used for treatment in patients with acute, decompensated heart failure. The efficacy of BNP monitoring, pre- and post-treatment with Natrecor, has been studied. Measurements of BNP two hours or more post-treatment detect only the endogenous levels of BNP.
 
Reference Value (ค่าอ้างอิง):
0 - 100  pg/mL
 
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s Reagent package insert Architect BNP, Abbott Laboratories, Diagnostic Division, Abbott Park IL 60064 USA.