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Test Code (รหัสการทดสอบ):
090-31-4016

Order Name (ชื่อการทดสอบ):
Bartonella Antibody Panel, IgG and IgM, Serum (MAYO, USA) **

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 2 tubes
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 14 days
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of Bartonella infection, especially in the context of a cat scratch
 
Methodology (วิธีการทดสอบ):
Immunofluorescence Assay (IFA)
 
Test List In Profile (การทดสอบใน Profile):
Bart Henselae IgG
Bart Henselae IgM
Bart Quintana IgG
Bart Quintana IgM
 
AliasesName (ชื่อเรียกอื่นๆ) :
Bartonella henselae Abs, IgG/IgM
Bartonella quintana, IgG/IgM
Bartonellosis
Cat Scratch Disease
BART
 
 
 
Test Code (รหัสการทดสอบ):
090-31-4016

Order Name (ชื่อการทดสอบ):
Bartonella Antibody Panel, IgG and IgM, Serum (MAYO, USA) **

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
N/A
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 2 tubes
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Serum, minimum volume 1 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Gross hemolysis: reject
Gross lipemia: reject
 
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8o 30 days
Serum Frozen 30 days

 
 
 
 
Test Code (รหัสการทดสอบ):
090-31-4016

Order Name (ชื่อการทดสอบ):
Bartonella Antibody Panel, IgG and IgM, Serum (MAYO, USA) **

 
Method detail (วิธีการทดสอบ):
Immunofluorescence Assay (IFA)
 
Schedule (ตารางการทดสอบ):
N/A **Sent out to MAYO, USA
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 14 days
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
MAYO Laboratory
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code (รหัสการทดสอบ):
090-31-4016

Order Name (ชื่อการทดสอบ):
Bartonella Antibody Panel, IgG and IgM, Serum (MAYO, USA) **

 
 
Clinical Information (ข้อมูลทางคลินิก):

Bartonella henselae and Bartonella quintana are small, rod-shaped, pleomorphic, Gram-negative bacteria. The human body louse (Pediculus humanis) is the proposed vector for B quintana. No animal reservoir has been determined for B quintana. The domestic cat is believed to be both a reservoir and vector for B henselae. Cats may infect humans directly through scratches, bites, or licks; or indirectly through an arthropod vector. Humans remain the only host in which Bartonella infection leads to significant disease.

The sight of entry for Bartonella is through openings in the skin. Microscopically, Bartonella lesions appear as rounded aggregates that proliferate rapidly. These aggregates are masses of Bartonella bacteria. Warthin-Starry staining has shown that Bartonella organisms can be present within the vacuoles of endothelial cells, in macrophages, and between cells in areas of necrosis. Occasionally organisms are seen in the lumens of vessels. While cutaneous lesions are common, disseminated tissue infection by Bartonella has been seen in the blood, lymph nodes, spleen, liver, bone marrow, and heart. B henselae has been associated with cat scratch disease (CSD), peliosis hepatitis (PH), bacillary angiomatosis (BA), and endocarditis. B quintana has been associated with trench fever, BA, and endocarditis. BA is a vascular proliferative disease usually involving the skin and regional lymph nodes.

CSD begins as a cutaneous papule or pustule that usually develops within a week after an animal contact. Regional lymphadenopathy follows and is the predominant clinical feature of CSD. Trench fever, which was a significant problem during World War I and World War II, is characterized by a relapsing fever and severe pain in the shins. PH and febrile bacteremia syndrome are both syndromes that have afflicted patients with AIDS and patients who are immunocompromised. While trench fever and CSD are usually self-limiting illnesses, the other Bartonella-associated diseases can be life-threatening.

Interest in B quintana and B henselae has recently increased since its increased prevalence in patients with AIDS, in transplant patients, and those with suppressed immunity.

 
Reference Value (ค่าอ้างอิง):

Bartonella henselae

IgG: <1:128

IgM: <1:20

Bartonella quintana

IgG: <1:128

IgM: <1:20

 
Interpretation (การแปลผล):

A positive immunofluorescence assay (IFA) IgM (titer >1:20) suggests a current infection with either Bartonella henselae or B quintana.

A positive IgG (titer >1:128) suggests a current or previous infection. Increases in IgG titers in serial specimens suggest active infection.

Normal serum specimens usually have an IgG titer of less than 1:128. However, 5% to 10% of healthy controls exhibit a B henselae and B quintana titer of 1:128. Sera from healthy volunteers rarely show titers of 1:256 or greater. IgM titers in normal serum are typically less than 1:20. IgM titers at 1:20 or greater have not been seen in the normal population.

Molecular testing of tissue for Bartonella species nucleic acid is recommended in cases of suspected endocarditis.

Cautions:
IgG cross-reactivity between Bartonella henselae and B quintana has been reported. However, the infecting species will usually have the higher titer.

A negative IgM result does not rule out infection.

Significant cross-reactions have been reported between Bartonella species and Chlamydia species.

 
Clinical Reference (เอกสารอ้างอิง):
www.mayomedicallaboratories.com (Retrieved: 22 Jun 2020)