This pseudo-enzymatic test contains organic peroxide and a cheomogen. The peroxidase effect of hemoglobin and myoglobin causes a color change to green.

Clues to glomerular origin of hematuria include lack of pain, brown-colored urine, proteinuria, red cell casts and dysmorphic red cells, hypertension, and evidence of renal dysfunction. The test will detect 0.03 mg/dL free hemoglobin or 10 intact red blood cells/μL. IgA nephropathy is the most common type of glomerular disease related to asymptomatic gross hematuria. In children, asymptomatic microscopic hematuria is often associated with hypercalciuria. In adults, >3.5 g of protein in a 24-hour urine collection provides indication of glomerular disease (Abuelo, 1983); >1 g of protein is suggestive. In children, random urine protein:creatinine ratio >2.0 is suggestive of glomerular disease.