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Clear
 
Test Code (รหัสการทดสอบ):
090-40-0091-01

Order Name (ชื่อการทดสอบ):
Bronchial Brush C/S

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Bronchial Brush/ Sterile container
* Place brush in sterile container with 1 mL. of saline
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of lower respiratory bacterial infections including pneumonia
 
Methodology (วิธีการทดสอบ):
1. Conventional Culture Technique
2. Identification by MALDI-TOF
3. Suceptibility test by Vitek 2 Compact
 
AliasesName (ชื่อเรียกอื่นๆ) :
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0091-01

Order Name (ชื่อการทดสอบ):
Bronchial Brush C/S

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Have patient rinse his/her mouth with water immediately prior to specimen collection. This reduces the number of contaminating oropharyngeal bacteria.

Medications: Do not use antibiotics before collection of specimen
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Bronchial Brush/ Sterile container
* Place brush in sterile container with 1 mL. of saline
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Bronchial Brush, volume Entire collection (minimum 0.5 mL.)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen in formalin will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Bronchial brush Room temperature, 18oC to 25oC 2 hours
Bronchial brush Refrigerated, 2oC to 8oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0091-01

Order Name (ชื่อการทดสอบ):
Bronchial Brush C/S

 
Method detail (วิธีการทดสอบ):
1. Conventional Culture Technique
2. Identification by MALDI-TOF
3. Suceptibility test by Vitek 2 Compact
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0091-01

Order Name (ชื่อการทดสอบ):
Bronchial Brush C/S

 
 
Clinical Information (ข้อมูลทางคลินิก):
Common bacterial agents of acute pneumonia include: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Pseudomonas aeruginosa, and members of the Enterobacteriaceae. Clinical history, physical examination, and chest X-ray are usually adequate for the diagnosis of pneumonia, and antimicrobial treatment is typically based on these findings.

Culture of expectorated sputum is used by some for the evaluation of pneumonia, although controversy exists regarding this practice; both sensitivity and specificity of sputum cultures are generally regarded as poor (<50%). Specificity is improved by collecting expectorated purulent matter from the lower respiratory tract and avoiding mouth and oropharyngeal matter, thereby reducing contamination. Prior to culture, the specimen should be examined for the presence of WBCs (evidence of purulent matter) and a paucity of squamous cells (evidence of minimal contamination by oral matter).
 
Reference Value (ค่าอ้างอิง):
No growth or usual flora
 
Interpretation (การแปลผล):
Isolate and identify potentially aerobic pathogenic organisms. Some bacterial agents that cause lower respiratory infections (eg, Mycobacteria, Legionella species, Mycoplasma pneumoniae) are not detected by this assay
 
Clinical Reference (เอกสารอ้างอิง):
1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition.
    Washington DC: American Society for Microbiology; 2016
2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th Edition.
    Washington DC: ASM Press; 2015
3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)