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Overview
ข้อมูลทั่วไป Specimen
ข้อมูลสิ่งส่งตรวจ Performance
ข้อมูลการทดสอบ Clinical & Interpretation
ข้อมูลทางคลินิก
 
Test Code (รหัสการทดสอบ):
090-40-0260-01
Order Name (ชื่อการทดสอบ):
Eye C/S
 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Eye/ Media plate or Stuart transport medium or Sterile screw-cap tube

Conjunctiva
  1. Sample eye with  swab (premoistened with sterile saline) by rolling over each conjunctiva
  2. Swab directly onto media plate (Blood agar and Chocolate agar) or Stuart transport medium
  3. Smear for Gram stain may be prepared at time of collection. Roll swab over 1- to 2-cm area of slide
Vitreous fluid
  1. Prepare eye for needle aspiration of fluid
  2. inoculation into Sterile screw-cap tube or inoculation of fluid directly onto media plate

Corneal scrapings
  • See details from Corneal scrapings C/S
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 days.
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of bacterial infections
 
Methodology (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
AliasesName (ชื่อเรียกอื่นๆ) :
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0260-01
Order Name (ชื่อการทดสอบ):
Eye C/S
 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Medications: Do not use antibiotics before collection of specimen
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Eye/ Media plate or Stuart transport medium or Sterile screw-cap tube

Conjunctiva
  1. Sample eye with  swab (premoistened with sterile saline) by rolling over each conjunctiva
  2. Swab directly onto media plate (Blood agar and Chocolate agar) or Stuart transport medium
  3. Smear for Gram stain may be prepared at time of collection. Roll swab over 1- to 2-cm area of slide
Vitreous fluid
  1. Prepare eye for needle aspiration of fluid
  2. inoculation into Sterile screw-cap tube or inoculation of fluid directly onto media plate

Corneal scrapings
  • See details from Corneal scrapings C/S
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Eye, volume Entire collection
 
Sub Mission Container (ภาชนะส่งตรวจ):
Media plate or Stuart transport medium or Sterile screw-cap tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Eye
(Conjunctiva/ Vitreous fluid)		 Direct media plate Room temperature, 18oC to 25oC 15 minutes
Stuart transport medium Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0260-01
Order Name (ชื่อการทดสอบ):
Eye C/S
 
Method detail (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0260-01
Order Name (ชื่อการทดสอบ):
Eye C/S
 
 
Clinical Information (ข้อมูลทางคลินิก):
Eye infections are usually caused by bacteria, fungi and viruses. Bacterial conjunctivitis is more common in children. The most common bacteria are Haemophilus influenza, Streptococcus pneumoniae and Staphylococcus aureus.
 
Reference Value (ค่าอ้างอิง):
No growth or normal flora
 
Interpretation (การแปลผล):
Any microorganism found where normal flora is not present is considered significant and is reported. For specimens contaminated with the normal bacterial flora, bacteria that are potentially pathogenic are identified.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)