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Test Code (รหัสการทดสอบ):
090-70-1001-04

Order Name (ชื่อการทดสอบ):
Mycobacterium tuberculosis Complex & NTM, PCR (Bronchial wash)*

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Bronchial washing/Sterile Container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 2 days.
 
Useful For (ประโยชน์การทดสอบ):
Use for detection of Mycobacterium tuberculosis (MTB) and non-tuberculosis mycobacteria (NTM) in the suspected patient.
 
Methodology (วิธีการทดสอบ):
Multiplex real-time polymerase chain reaction (Real-Time PCR)
 
Test List In Profile (การทดสอบใน Profile):
Mycobacterium tuberculosis complex (MTB)
Non-tuberculosis mycobacteria (NTM)
 
AliasesName (ชื่อเรียกอื่นๆ) :
Acid-Fast Bacilli (AFB)
Mycobacterium tuberculosis (MTB)
Tuberculosis (TB)
PCR for MTB 
PCR for TB
PCR for Tbc
 
 
 
Test Code (รหัสการทดสอบ):
090-70-1001-04

Order Name (ชื่อการทดสอบ):
Mycobacterium tuberculosis Complex & NTM, PCR (Bronchial wash)*

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Bronchial washing/Sterile Container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Bronchial washing (BW) 10-20 mL (Minimum volume = 1.5 mL)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile Container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen is added any preservative solution.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Bronchial washing Refrigerated, 2oC to 8oC 3 days
 
 
 
Test Code (รหัสการทดสอบ):
090-70-1001-04

Order Name (ชื่อการทดสอบ):
Mycobacterium tuberculosis Complex & NTM, PCR (Bronchial wash)*

 
Method detail (วิธีการทดสอบ):
Multiplex real-time polymerase chain reaction (Real-Time PCR)
 
Schedule (ตารางการทดสอบ):
Test daily, at 07:00 p.m.
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 2 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-70-1001-04

Order Name (ชื่อการทดสอบ):
Mycobacterium tuberculosis Complex & NTM, PCR (Bronchial wash)*

 
 
Clinical Information (ข้อมูลทางคลินิก):
Tuberculosis is an infectious bacterial disease caused by Mycobacterium tuberculosis and is known to spread through the air. When infected persons cough, spit, or talk, the M. tuberculosis organisms are dispersed into the air. Only a small number of M. tuberculosis is enough to cause an infection when inhaled. However, not all people infected with M. tuberculosis will become sick; the immune system either kills or walls off the germs where they can lie dormant for years. Failure of the immune system to control infection with M. tuberculosis leads to active disease. Tuberculosis and acquired immunodeficiency syndrome (AIDS) form a lethal combination. AIDS weakens the immune system, and a person that is human immunodeficiency virus (HIV)-positive and infected with M. tuberculosis is much more likely to become sick with tuberculosis than someone who is HIV-negative and infected with M. tuberculosis.
 
Reference Value (ค่าอ้างอิง):
Test Reference Value
Mycobacterium tuberculosis complex (MTB) Negative
Non-tuberculosis mycobacteria (NTM) Negative
 
Interpretation (การแปลผล):
  1. This test does not differentiate among members of the M. tuberculosis complex, i.e., M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, M. microti, M. canetti, and M. pinnipedii.
  2. A negative result does not preclude the presence of MTB infection or MDR-TB, should be confirmed by mycobacterial culture.
  3. A positive result does not necessarily indicate the presence of viable organisms. It indicate the presence of the MTB DNA but cannot distinguish between viable, disease-related organisms and nucleic acid persisting from prior infection. The agent detected may not be the definite cause of disease. The use of additional laboratory testing (e.g. bacterial and viral culture, immunofluorescence, and radiography) and clinical presentation must be taken into consideration in order to obtain the final diagnosis.
  4. Reliable results are dependent on adequate specimen collection, transport, storage and processing procedures.
  5. The detection of MTB and NTM is dependent on the number of organisms present in the specimen and may be affected by specimen collection methods, patient factors, stage of infection and/or infecting Mycobacterium strain.
 
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s package insert, AnyplexTM MTB/NTM Real-time Detection (V2.0), January 2019, Seegene, Inc. Seoul 138-050, Korea.