1. Two sets of disposable breath bags.
2. Plain water 100 milliliters.
3. UBIT tablet 100 milligrams.

1. Avoid antibiotics and bismuth subsalicylate for at least 4 weeks before the test.
2. Avoid sulcralfate (Ulsanic^®) for at least 2 weeks before the test.
3. Avoid proton pump inhibitors (PPI) for at least 2 weeks before the test:
   • Omeprazole (Losec^®)
   • Esomeprazole (Nexium^®)
   • Rabeprazole (Pariet^®)
   • Pantoprazole (Controloc^®)
   • Lansoprazole (Prevacid^®)
4. Avoid Potassium-Competitive Acid Blockers (P-CABs) e.g. Vonoprazan (Vocinti^®) for at least 5 days before the test.
5. Avoid food and drink for at least 4 hours before the test.
6. Avoid alcoholic beverages and beverages that contain urea, such as sugarcane juice and pineapple juice, for at least 4 hours before the test.
7. Avoid smoking for at least 4 hours before the test as smoke in the lungs can interfere with the measurement of carbon dioxide.

1. Blow into the first bag until it is fully inflated.
2. Swallow the UBIT tablet within 5 seconds and drink one glass of water (100 milliliters). Do not chew the table or grind it up or let it dissolve.
3. Lie on your left side for approximately 5 minutes.
4. Sit up for 15 minutes.
5. When 20 minutes have passed since the UBIT tablet was swallowed, blow into the second bag until it is fully inflated.
6. Submit both bags to a hospital officer for analysis.
7. You will be asked to return for the results of the test.

1. The UBIT must not be chewed, grounded or dissolved when it is taken.
2. While blowing into the bag, be sure to place the bag against your mouth. Take a deep breath in through your nose (5 to 10 seconds) and then blow out into the bag to ensure the highest concentration of carbon dioxide for analysis.
3. Blow out as slowly as you can. If you are not able to take and hold deep breaths, just repeat the inhale/exhale process. The bag is equipped with a one-way valve that prevents backflow.

Attach the test bags before and after taking medication in the positions specified on the ¹³C Urea Breath Analyzer POCone analysis machine and press “Enter.” The machine takes approximately 2 minutes and 30 seconds to analyze the results. The machine will then print out the results to show the difference between the two bags, one before swallowing the UBIT tablet and one after. It will also display a “positive” or “negative” result.

• Positive (+) means there is bacteria.
• Negative (-) means there is no bacteria.